Hela vimentin
WebDec 1, 1992 · Using both vimentin-expressing (HeLa) and vimentin-negative (MCF-7) epithelial cell lines, we have identified the cis-regulatory DNA elements involved in this process. Sequences located 1.1-0.6 kb upstream of the vimentin transcription-initiation site strongly enhance expression in HeLa cells, but are silenced in MCF-7 cells. WebThe absence of signal in the vimentin knockout HeLa cells confirms specificity of the antibody for vimentin. Western blot analysis of extracts from various cell lines using …
Hela vimentin
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Vimentin was one of the CCAT1 binding proteins and was participated in the tumor metastasis pathway. Knocked down VIM and rescued the downregulation by overexpressing CCAT1 demonstrated that CCAT1 could enhance tumor migration and invasion abilities by stabilizing Vimentin protein. WebRecognizes endogenous levels of Vimentin protein. Clonality. Monoclonal. Dilution. WB (1/1000 - 1/3000), IHC (1/200 - 1/500), IF/IC (1/100 - 1/200) Gene Symbol. VIM. Storage Condition. Store at -20°C long term. MA9220. 货号 规格 价格(￥) MA9220M 100μl MA9220S 50μl 以上产品均有现货供应. 订购咨询： orders@ab-mart ...
WebSimilar results were obtained using bacterially purified His-tagged Rab7a wt and mutant proteins, in order to pull-down endogenous vimentin from HeLa total extracts (Fig. 2 C–D). Indeed, CMT2B-causing Rab7a mutant proteins were able to pull-down vimentin about 2 fold more efficiently than Rab7a wt (Fig. 2 D). WebWestern blot analysis of extracts from control HeLa cells (lane 1) or vimentin knockout HeLa cells (lane 2) using Vimentin (V9) Mouse mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the vimentin knockout HeLa cells confirms specificity of the antibody for vimentin. Show More
WebMar 31, 2024 · At 8 hpi, cells were fixed, nuclei and replication sites were stained with Hoechst and immunostaining was performed to visualise vimentin. In untreated HeLa cells, vimentin was associated with replication sites and newly assembled virions were found throughout the cytoplasm (Figure 3e). As expected, in acrylamide-treated HeLa cells … WebSimilar results were obtained using bacterially purified His-tagged Rab7a wt and mutant proteins, in order to pull-down endogenous vimentin from HeLa total extracts (Fig. 2 …
WebThe absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin. Show More Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using Vimentin (D21H3) XP ® Rabbit mAb #5741. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.
WebThe absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin. Data were generated using the standard formulation of this product. Show More Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP ® Rabbit mAb. eakin trioWebab154207 staining Vimentin in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab154207 at a working dilution of 1 in 100 (shown in pseudo color red) ... csom tuitionWebThe absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin. Show More Simple Western™ analysis of lysates (1 mg/mL) from … csom update webpart propertiesWebAfter being transfected and screened with puromycin, it was found that compared with the HeLa cell controls, both mRNA level and protein expression of vimentin in the transfected HeLa cells were significantly decreased ( Figure 1 B,C), and vimentin knocked out cells were named KO-vimentin HeLa cells, abbreviated as KO HeLa cells. cso multiplex chicagoWebMar 20, 2024 · To further investigate whether vimentin is a functionally relevant target of ajoene’s anti-metastatic activity, we transiently overexpressed vimentin in HeLa and MDA-MB-231 cells (Fig. 6a). A scratch wound was then introduced into the cell monolayer, followed by addition of 10 μM Z-ajoene in DMSO or DMSO alone for 24 h. csom update list itemWebVimentin was one of the CCAT1 binding proteins and was participated in the tumor metastasis pathway. Knocked down VIM and rescued the downregulation by overexpressing CCAT1 demonstrated that CCAT1 could enhance tumor migration and invasion abilities by stabilizing Vimentin protein. eakin toronto gtahttp://www.ab-mart.com.cn/page.aspx?node=%2077%20&id=%2053980 csom vip face ids